【摘要】 采用改进的乳酸 - PAGE技术对不同玉米 (Zea mays)自交系和杂交种种子的白蛋白和球蛋白进行了系统电泳分析 ,发现此方法具有高度的分辨力和稳定性。把电泳图依其迁移率分为 α、β、γ和 ω4个带区。各自交系和杂交种的电泳图均有其鲜明特征 ,彼此可以区别 ,视作它们各自的“指纹”。全籽粒电泳谱带与胚的电泳谱带基本相同。胚乳产生谱带少而弱 ,且大都和胚的谱带发生重叠。亲本自交系的不同谱带在杂种 F1 发生互补。杂种谱带等于两亲本共同谱带与各自独有谱带之和。而且正、反交 F1 谱带以及它们两亲本种子提取液机械混合产生的谱带完全相同 ,表明谱带的差异是一种由核基因控制的遗传性状。F1 的谱带类型可由两亲本谱带类型预测 ,也可由两亲本种子提取液混合而产生的电泳图所演示更多还原

【Abstract】 Systematic electrophoretic analysis of albumins and globulins of the inbred and hybrid corn (Zea mays) seeds was carried out on an improved lactate polyacrylamide gel electrophoresis, a method with high resolving power, good reproducibility and stability. The electrophoregram was classified into four groups designated as α,β,γ,and ω respective ly. Each inbred or hybrid had its own unique band pattern distinguishable from the others, regarding as its “fingerprint”. The band pattern of the whole kernel was basically similar to that of its embryo, except that of the endosperm showing less bands with weaker staining intensity; and most of the patterns overlapped with those of the embryo. The band number of the F 1 hybrid was exactly equivalent to the number of the common bands and the specific bands of the two parents, indicating that the difference of band patterns was a genetic trait controlled by the nuclear genes. The F 1 electrophoregram could be predicted by those of the two parents. The band pattern of the F 1 hybrids was identical with that produced from me chanically mixed extract of the two parent inbreds. This procedure could be used in corn cultivar identification and as a test for genetic purity.更多还原