【摘要】 查尔酮合酶 ( chalcone synthase,CHS)是花色素合成途径中的一个关键酶 ,它在植物中表达量的改变可能影响花的颜色。从矮牵牛 ( Petunia hybrida)特定发育阶段的花瓣的 c DNA中 ,克隆到查尔酮合酶基因 ,并正向插入到原核表达载体和含有花椰菜花叶病毒 Ca MV 35 S启动子的真核表达载体中 ,在原核中得到高效表达 ,并通过土壤农杆菌介导的方法转化矮牵牛。转基因植物的花色不但发生了明显的变异 ,其育性也受到了影响 ,不能产生正常花粉粒 ,成为雄性不育植株。 Northern杂交表明 ,转基因植物花瓣中 ,内源及外源查尔酮合酶基因转录均受到抑制更多还原

【Abstract】 Chalcone synthase A (CHSA) is a key enzyme in the biosynthesis of all classes of flovonoids, and variation of its expression might affect the colour of flowers. CHSA gene was cloned from flower petals just coming into bloom and inserted in procaryotic expression vector and eucaryotic vector which contains CaMV 35S promoter in a sense orientation. CHSA gene was highly expressed in procaryotic expression system. Petunia (Petunia hybrida) leaf discs were transformed by Agrobacterium tumefaciences LBA4404. Not only flower colours of the resulted transgenic plant were altered, but the transgenic plants became male sterile. Northern blotting assays showed that the transcription of both transgene and endogenous genes were suppressed in the flower petals of transgenic plant.更多还原