【摘要】 利用发根农杆菌 (Agrobacterium rhizogenes)菌株 R1 60 1 ,成功地从药用植物栝楼 (Trichosantheskirilowii Maxim.)中诱导出发根并建立了栝楼发根的离体培养系统。通过蛋白质印迹法对栝楼发根的天花粉蛋白进行了鉴定。利用 SDS- PAGE和 TLC扫描 ,测定出每克鲜重栝楼发根中天花粉蛋白的含量可达 8.1 6mg。更多还原

【Abstract】 Hairy roots of Trichosanthes kirilowii Maxim. were successfully induced by infection with Agrobacterium rhizogenes strain R1601 and an in vitro culture system of the hairy roots was established.The presence of trichosanthin in the hairy roots of T. kirilowii was confirmed by western blotting.The yield of trichosanthin was determined to be 8.16 mg/g fr.wt with SDS PAGE gel electrophoresis and thin layer chromatography.更多还原