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D1/D2/Cytb-559复合物的共振拉曼光谱

THE RESONANCE RAMAN SPECTRA OF β-CAROTENE MOLECULES IN THE PHOTOSYSTEMⅡ REACTION CENTER D1/D2/Cyt b 559 COMPLEX *

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【作者】 卢荣禾匡廷云于振宝唐崇钦汤佩松李庚新唐树延

【Author】 Lu Rong he, Kuang Ting yun, Yu Zhen bao, Tang Chong qin and Tang Pei song (Institute of Botany, Academia Sinica, Beijing 100093) Li Geng xin and Tang Shu yan (Changchun Institute of Physics, Academia Sinica, Changchun 130021)

【机构】 中国科学院植物研究所!北京100093中国科学院长春物理研究所!长春130021

【摘要】 光系统Ⅱ(PSⅡ)反应中心D1/D2/Cytb-559 复合物在488 nm 处激发的共振拉曼光谱显示4 个主要谱带,其峰位分别在1532(υ1)、1165(υ2)、1010(υ3)和970(υ4) cm - 1处,表明PSⅡ反应中心结合的β-胡萝卜素分子是全反式构型。D1/D2/Cytb-559 复合物的色素抽提液的拉曼光谱也显示4 个主要的拉曼峰,其中υ4 谱带的强度急剧下降,说明PSⅡ反应中心内部结合的β-胡萝卜素分子与抽提液中自由的β-胡萝卜素分子的构象不同,而与光合细菌反应中心内部的类胡萝卜素分子的构象相似,其共轭多烯链的平面也处于扭曲状态

【Abstract】 The resonance Raman spectrum of β carotene in photosystem Ⅱ(PSⅡ)reaction center complex was characterized by four main bands, peaking at 1532(υ1), 1156(υ2), 1010(υ3)and 970(υ4)cm -1 , respectively, with several additional small Raman bands in the region between 1100 cm -1 and 1500 cm -1 .It was suggested that β carotene molecules of the reaction center complex were in all trans configuration.The resonance Raman spectrum of an acetone extract from the reaction center complex also showed four main bands.The peak position of υ1, υ3 and υ4 band shifted 5 cm -1 to the shorter wave number.The most dramatic changes were the reduction of the intensity of υ4.From the above results it was demonstrated that the conformation of β carotene molecules in the PS Ⅱreaction center was not the same as that of free β carotene molecules in solution, but similar to that of carotenoid molecules in the photosynthetic bacterial reaction center, in other words, they are likely to be in a twisted conformation.

  • 【文献出处】 植物学报 ,Acta Botanica Sinica , 编辑部邮箱 ,1996年03期
  • 【分类号】Q946.8
  • 【被引频次】9
  • 【下载频次】48
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