【摘要】 用ＲＴ－ＰＣＲ技术从ＰＲＶ－ＡＬ中分离到复制酶（ＲＰ）基因，将基因克隆进载体ｐＵＣ１８，用双脱氧链终止法测定了基因序列，表明其全长为１６０２ｂｐ，与国内外报道的ＨＡ５－１、ＹＫ和Ｓｍ的ＲＰ基因相比，同源性分别达８２．８０％，９５．０７％和９１．８３％．更多还原

【Abstract】 The replicase (RP)gene of abnormal leaf isolate(AL) of papaya ringspot virus (PRV)was synthesized by reverse transcription (RT) and polymerase chain reaction (PCR)amplification, and cloned into pUC18. The gene was sequenced by the dideoxy chain termination method. Results showed that it was 1 602 bp in length, and shared 82 80%,95 07% and 91 83% homologies with ones of the strains HA5 1, YK and Sm respectively.更多还原