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金鱼草下胚轴不定芽发生及细胞学观察

Generation and Cytological Observation of Adventitious Buds from Hypocotyl Explants of Antirrhinum majus L.

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【作者】 余迪求邓庆丽沈亚楠李宝健

【Author】 Yu Diqiu Biotechnology Research Centre, Zhongshan University, Guangzhou 510275 Deng Qingli Sheng Yanan Li Baojian

【机构】 中山大学生物工程研究中心

【摘要】 采用金鱼草下胚轴为外植体,培养于附加不同激素浓度组合MS固体培养基上.其外植体对激素的反应表现出很大的差异.在附加2,4-D培养基上,下胚轴可诱导形成淡基色愈伤组织,但不易分化;培养于附加BA培养基上,外植体可诱导产生丛生不定芽.将不定芽转移至附加NAA培养基上,可诱导根的形成.细胞学观察证实下胚轴不定芽的发生起源于表皮细胞

【Abstract】 The hypocotyl explants from Antirrhinum majus L. were cultured on MS agar medium with different plant growth regulator concentrations and combinations. Large differences were found in the responses of the hypocotyl explants. On the medium with 2,4 D(1~2 mg/L), the explants were found to produce healthy, viable and yellowish calli which did not differentiate to generate buds; but on the medium with 6 BA(2 mg/L) and NAA(0.2~0.5 mg/L),the explants produced healthy plantlets and yellowish calli. Shoots formed from the buds on MS agar medium supplemented with 6 BA(0.5~2 mg/L) and NAA(0~1 mg/L).These shoots, if transferred individually to MS agar medium with NAA(0.5~2 mg/L), produced roots at their bases. On the medium with NAA 0.5 mg/L, the roots of plantlets were healthy. Cytological observations indicated that the plant regeneration originated from epidermic cells of the explants.

【基金】 中山大学“211工程”基础性研究前沿基金资助项目
  • 【文献出处】 中山大学学报(自然科学版) ,ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI , 编辑部邮箱 ,1996年04期
  • 【分类号】Q945.52
  • 【被引频次】8
  • 【下载频次】109
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