【摘要】 筛选两株生长快、产木聚糖酶活力高的菌种；黑曲霉（Ａｓｐｅｒｇｉｌｌｕｓｎｉｇｅｒ，ＡＮ０１）、链霉菌（Ｓｔｒｅｐｔｏｍｙｃｅｓｓｐ．Ｓｔｒ７Ｂ），酶活力分别达１２８ｍｇ／Ｌ·ｍｉｎ和１７６ｍｇ／Ｌ·ｍｉｎ；酶反应最适温度分别为６０℃与５０℃；最适ｐＨ值为５．０和６．０，并分别在ｐＨ２．２～５．０，５．８～６．４酶活性稳定；在６０℃条件下保温１．５ｈ，酶活力分别剩余２０．５％，８８．５％，其中ＡＮ０１株原酶液在９０℃保温１０ｍｉｎ，活力仍剩余１４．５％．Ｃｕ２＋对酶活表现出极强抑制，Ｆｅ２＋，Ｍｇ２＋，Ｃａ２＋等离子则有促进作用；用纸层析法探讨了不同培养时间各种产物产生的情况．更多还原

【Abstract】 Two strains of Aspergillus niger (AN01) and Streptomyces sp. (Str7B) with fast growing and high xylanase activity were isolated from soil samples. The enzyme activity of AN01 was 128 mg/L. min, at 30℃ for 72h. with shaking and that of Sir7B was 176mg/L. min, at 50℃ for 72h. The optimal temperature and pH for xylanase reaction were 60℃ and 5.0 for AN01 and 50C, 6. 0 for Sir7B. The enzyme activity of AN01 was stable within PH2. 2-5.0 and that of Sir7B was PHS. 8-6.4. The remaining activity of AN01 and Sir7B xylanase were 20. 5% and 88. 5% after incubation at 60℃ for 1.5h, Fe2+, Mg2+ and Ca2+ increased both of the activities whereas Cu2+ inhibited them strongely. The products of enzyme hydrolysis in different culture times shown bymeans of paper chromatography were also discussed.更多还原