【摘要】 ＥＭＳ诱变的细胞经过Ｂｒｄｕ递增浓度的定向筛选，建立了可以抗３００ｍｇ／Ｌ浓度Ｂｒｄｕ的斜纹夜蛾胸苷激酶缺陷型细胞株．经［３Ｈ］胸苷磷酸化反应测定胸苷激酶（ＴＫ）活力表明，所建立的Ｓｌ－ＴＫ－细胞株已经不具备ＴＫ活力更多还原

【Abstract】 A Spodoptera litura cell line deficient in thymidine kinase activity (Sl TK -) was isolated by mutagenesis with Ethylmethanesulfonate (EMS) and serial passages in the presence of 5’ bromodeoxyuridine(Brdu) at stepwise increasing concentrations. The resulted Sl TK cell line was confirmed to be lacking in TK activity by unable to catalyze the phosphorylization of thymidine.更多还原