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养殖斑节对虾红体病病原及组织病理研究
Study on Pathogen and Histopathology of Red Disease of Cultured Penaeus monodon
【摘要】 养殖班节对虾红体病病原经组织病理、电镜观察和白斑杆状病毒PCR检测,初步证实是一种枝原体.枝原体高度多态性,主要以球状体和分枝丝状体形式存在.球状体直径60~108um,分枝丝状体膨大部直径达150um,丝状部为25um.枝原体主要寄生在斑节对虾肝胰腺上皮细胞、造血组织细胞、鳃上皮细胞、甲壳表皮细胞等,致肝胰腺萎缩糜烂.受感染细胞细胞质空泡变性,细胞核固缩解体,内质网、高尔基体和溶酶体消失.对红体带白斑斑节对虾、白斑(无红体特征)斑节对虾和红体(无白斑特征)斑节对虾组织病理、电镜和白斑杆状病毒PCR检测,证实斑节对虾红体病不是由白斑杆状病毒引起,红体斑节对虾具有白斑杆状病毒是病毒继发感染所致.
【Abstract】 Hepatopancreas, gill, epidermis, antennal gland, gut and hematopoietic tissue of cultured Penaeus monodon with red disease were observed by light and electron mi-croscopy. We found a mycoplasma in hepatopancreas of all P. monodon with red disease, bacteria which were detected by cultured methods and white spot syndrome baculovirus which was detected by polymerase chain reaction were found in one third P. monodon with red disease, respectively. We think that mycomplasma is one of the pathogen that cause red disease of cultured P. monodon.
【Key words】 Penaeus monodon; red disease; mycoplasma; white spot syndrome baculovirus; bacteria;
- 【文献出处】 中山大学学报论丛 ,Supplement To The Journal of Sun Yatsen University , 编辑部邮箱 ,1996年S1期
- 【分类号】Q947
- 【被引频次】6
- 【下载频次】180