【摘要】 对７个菌属１５１０株肠道杆菌进行了大肠菌素检测，宋内氏菌Ｃｏｌ质粒检出率最高为７８．１８％，其次为普通大肠杆菌３３．４７％，福氏志贺氏菌、克雷伯氏菌、伤寒沙门氏菌分别为８．０％、３．５％、２．３２％，不同种属菌株大肠菌素检出率差异悬殊。药敏试验证明，９０．５％（１０５／１１６）Ｃｏｌ＋大肠杆菌具有耐药性，在接合实验中，２６．２％（１６／６１）大肠杆菌能传递Ｃｏｌ质粒。将Ｃｏｌ＋菌株接种保种半固体置室温２年，１９．９％（３１／１５６）的菌株丢失Ｃｏｌ质粒。本实验同时建立一种直接覆盖指示菌──插种受检菌以检测大肠菌素的方法。通过大量菌株考核，结果与Ｍｏｎｋ法基本一致，而且比后者简单、快速。在细菌素的调查研究中具有广泛的应用价值。更多还原

【Abstract】 From 1510 strains of Enterobacteria Col plasmids detected belonged to 7 genus.As a result,the positive rates of Col plasmids from Sh. sonnei were the highest(78.1%),E.coli second(33.5%),Sh.flexneriK.pneumoniae,and S.typhosa 8.0%,3.6%,2.3%,respectively.Identified by drug sensitivity,90.5% of Col+ strains of E.coli were resistant to antibiotics.The results also showed that 26.2% of the Col plasmids harboring in E. coli could transfer to E.coli K12.After being kept for two years, 19.9% of Col+ strains examined lost their Col+plasmids.In addition a new method to dctect colicim was formed, in which the agar medium was covered with the indicated bacteria first and then the bacteria examined were stabbed into the medium,termed overlayer-stab method.After examined were strains,the result of this method showed that it was in agreement with that of Monk’s(stab-overlayer method).The overlayer-stab method was not only simpler but also quicker and cheaper than Monk’s.Thersfore the new method can be used widely in study of colicin.更多还原