【摘要】 用ＲＡＰＤ引物Ｊａｓ１５从亲本ＣＴ９９９３中扩增出的多态性产物１５ｋｂ片段，经ＮｃｏⅠ酶切后获得的一条０５ｋｂ片段，将其制备成ＲＦＬＰ探针ｊａｓ５００。选用覆盖整个水稻ＲＦＬＰ图谱的１２５个随机克隆和ｊａｓ５００对ＣＴ９９９３／ＫＤＭＬ１０５的Ｆ２群体进行检测，结果表明：ＫＤＭＬ１０５的香味遗传受一对隐性基因控制；控制香味基因ａｒｏ与ｊａｓ５００以及第８染色体上的几个ＲＦＬＰ标记有连锁关系，并将其初步定位于第８染色体上ｊａｓ５００和Ｃ２２２之间，距离分别为１５８ｃＭ和２７８ｃＭ，该香味基因在ＲＦＬＰ图谱上的初步定位为进行该基因的精细定位及有效利用奠定了基础，ＲＦＬＰ标记ｊａｓ５００和ＲＡＰＤ引物Ｊａｓ１５均可直接应用于水稻育种实践。更多还原

【Abstract】 A 0 5 kb fragment was obtained by digesting with restriction enzyme NcoⅠ from the 1 5 kb band amplified by RAPD primer Jas 1 5 in CT9993 and developed into an RFLP marker named jas 500 Aromatic evaluation in the progenies of the cross indicated the presence of a major gene in KDML 105 A recessive locus for jasmine-like aroma in KDML 105 was detected and mapped between RFLP markers jas 500 and C222 on chromosome 8 The distance between jas 500 and aro gene was estimated to be 15 8 cM, and C222 is on the other side of aro gene at a distance of 27 8 cM Closely linked RFLP marker jas 500 as well as RAPD marker Jas 1 5 may facilitate early selection for aroma gene in rice breeding programs.更多还原