【摘要】 应用商品化酶联免疫吸附试验（BLISA）试剂盒和血凝抑制试验（HI）法,同时测定了173个常规鸡产蛋下降综合症(EDS₇₆)抗体送检样品.结果显示,ELISA 法的灵敏度和特异性分别为 HI 法的93.9%和100%.每一样品用这二种方法在相互独立的条件下,检测取得了基本相同的结果,Kappa 值达到了0.97,表明了这二种方法有很好的一致性。二种方法抗体效价的相关性分析表明,相关系数为0.77.在对带有高、中、低抗体和阴性混合血清的重复测定中,发现随着 S/P 值的变小,其标准差也随之减小,显示了较好的重复性.本试验还对阴阳性判别的临界点的设定对试验灵敏度的影响进行了分析。更多还原

【Abstract】 An assay of 173 routine chicken serum samples for egg drop syndrome(EDS)antibodies was determined the a commercial EDS enzyme-linked immunosorbent assay(ELISA).It had a 93.9% sensi- tivity and 100% specificity relative to EDS haemagglutination inhibition(HI)test.The correlation coefficient of antibody titers berween two tests was 0.77.For individual,two tests can have the same result based upon chance alone.The Kappa value between the ELISA and HI test was calculated to be 0.97, which indicates a highly significant agreement between two tests.The ELISA repeated test of the four se- rum pools from high,medium,low titer antibodies and negativeflocks and showed consistent results.In the test,the influence on sensitivity and specificity of ELISA by adjusting cut-offpoint were analysed too.更多还原