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环胞苷和丙氧鸟苷联合应用的抗单疱病毒作用

Anti-herpes simplex virus action of combined therapy with cyclocytidine and ganciclovir

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【作者】 陈祖基宋洁贞陈慷谢雷克陈悦高长凤

【Author】 Chen Zuji Song Jiezhen Chen Kang Henan Institute of Ophthalmology,Zhengzhou 450003

【机构】 河南省眼科研究所河南省眼科研究所

【摘要】 目的研究环胞苷和丙氧鸟苷联合用药的抗单疱病毒(herpes simplex virus,HSV)作用;方法用空斑抑制试验测定环胞苷和丙氧鸟苷单独或联合用药后抗 HSV 的半数抑制浓度(50%inhibitive concentration of HSV plaque formation,IC50),应用图解法和分部抑制浓度(fractionalinhibitory concentration,FIC)指数,评价环胞苷和丙氧鸟苷联合应用的抗 HSV 活性;结果在组织培养中,环胞苷和丙氧鸟苷抑制 HSV-1的 IC50分别为0.19μg/ml 和0.10μg/ml。当环胞苷和丙氧鸟苷联合应用时,抗 HSV 活性有明显协同作用,FIC 指数均小于0.75。环胞苷和丙氧鸟苷合用尚能减轻及延缓 HSV 耐药性的产生;结论环胞苷和丙氧鸟苷联合应用可能是治疗 HSV 眼部感染的一种有效方法.

【Abstract】 Objective The study was designed to investigate the combined effect of cyclocytidine (CC) and ganciclovir (GCV) on herpes simplex virus-1 (HSV-1) in cell culture.Methods The 50% inhibition concentrations of HSV-1 plaque formation (IC50) of CC,GCV alone and in combination were determined by the inhibitory test of plaque formation.The combined anti-HSV-1 effect of CC and GCV was evaluated by a graphic method and fractional inhibitory concentration (FIC) indexes.Results IC50 of CC and GCV was 0.19 and 0.1μg/ml,respectively.The combination of CC with GCV produced sig- nificantly synergistic activity against HSV-1 in cell culture.FIC indexes were all below 0.75.The com- bined therapy of CC and GCV can also decrease and delay the emergence of drug-resistant variants. Conclusion These results suggest that this combined therapy of CC and GCV may be a potentially ef- fective means in the management of patients with HSV-1 ocular infection.

  • 【文献出处】 中华眼科杂志 ,Chinese Journal of Ophthalmology , 编辑部邮箱 ,1996年01期
  • 【分类号】R96
  • 【被引频次】5
  • 【下载频次】22
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