【摘要】 目的通过对1992～1994年院内感染的1135株革兰阴性杆菌对头孢菌素的耐药分析,指导临床合理用药。方法应用 VITEK AMS-60自动微生物分析仪、K-B 法对菌株进行鉴定、药敏试验,并按美国临床实验室标准委员会(NCCLS)标准分析、质控。结果药敏质控符合 NCCLS 标准;1135株革兰阴性杆菌对头孢呋肟、头孢哌酮等耐药性检测,均经 U 检验,P<0.01,表明耐药性明显增加。结论随头孢菌素广泛使用,其耐药性逐年上升,应引起临床重视。更多还原

【Abstract】 Objective The analysis of the resistance to cephalosporin of 1135 gram-negative(G) bacilli from nosocomial infection from 1992 to 1994 is useful to instruct rational application of cephas- porin in clinical work.Methods Bacteria were identified with VITEK AMS60.The susceptibility were assessed according to NCCLS standard.The susceptibility test were mainly finished on VITEK AMS60 and the others were done by K-B method.Results In susceptibility test,results of standard strains of bacteria accorded with NCCLS standard.The resistance to CFX and CPZ from 1135 G bacilli signifi- cantly increased(P<0.01).The occurrence of resistance to cephalosporin increased in a few species of common bacilli including Klebsiella pneumoniae,etc.The resistance to antibiotics of 311 strains of G- bacilli in respiratory tract infection also increased with time.Conclusions Because resistance to antibi- otics increased year by year,it should be paid great attention by clinicians.更多还原