【摘要】 为了解结直肠癌ｐ５３基因突变的情况，应用聚合酶链反应－单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术对３０例人结直肠癌中ｐ５３基因第５、６、７、８外显子进行检测，发现１６例（５３．３３％）存在ｐ５３基因突变，其中第７外显于７例，第５外显子４例，第６外显子３例，第８外显子２例。结果表明：（１）结直肠癌中普遍存在ｐ５３基因突变；（２）ｐ５３基因突变与结直肠癌的发生和发展有关；（３）ＰＣＲ－ＳＳＣＰ银染技术是一种敏感、可靠且简便、快速和经济的方法，尤其适用于临床实验室进行大规模的样本筛选。更多还原

【Abstract】 Using PCR-SSCP sliver staining technique,we analyzed 30 patients with colorectal cancer for mutation in p53 gene exons 5,6,7 and 8.16 out of 30 cases tested were p53 gene mutation positive which yielded a total mutation rate of 53.33%. It was found that there were 7 cases(43.75%) of exon 7,4 cases(25.00%) of exon 5,3 cases(18.75%) of exon 6,and 2 cases(12.50%) of exon 8. Our results showed that p53 gene mutation is a common event in colorectal cancer,which plays an important role during colorectal carcinogenesis,and PCR-SSCP sliver staining approach is one of the most sensitive and simplest measurement for detecting genetic mutation. It is worth using in clinical laboratory because of its readiness and lower cost.更多还原