【摘要】 本文分析了３０例正常人ＰＲＬ经ＰＨＡ活化的Ｔ细胞对ＩＬ－２及ＩＬ－４的增殖应答特点；及整合有人ＩＬ－４受体ｃＤＮＡ的ＣＴＬＬ－２／ＨＩＬ－４Ｒ细胞系对人ＩＬ－４的特异增殖应答特点。结果表明，正常人经ＰＨＡ活化的ＰＢＬ对ＩＬ－２有良好增殖应答曲线者占９３％，而对ＩＬ－４有良好增殖应答者只占２０％。　ＣＴＬＬ－２／ＨＩＬ－４Ｒ细胞系对人ＩＬ－４呈规律性增殖应答曲线，且应答敏感性高，可测出０．０９ｕ／ｍｌ，可推荐作为测定人ＩＬ－４生物活性的一种准确方法。更多还原

【Abstract】 Human Interleukin 4 (hlL-4 ) is a cytokine with important immuno-regulating activities. Precise assessment of hlL-4 activity become. increasingly critical. We reported the characteristics of proliferative response of 30 samples of PHA activated T cells in PBL obtained from normal human donors to rhlL-2 and rhlL-4. The hlL-4 bioactivity was also assessed by its support of the proliferation of CTLL-2/HIL - 4R cell line ,a CTLL-2 derived cell line integrated with hlL -4 receptor CcN A . Through statistical analysis , our results showed that 93% of the samples of PHA activated PBL responded well to IL -2 by proliferation with a positive correlation of dose response. In contrast ,only 20% of these samples showed good dose-response curve to hlL-4 ,The sensitivity of IL-2 and IL -4 is 0. 5U /ml and 8. 2U /ml respectively. As expected , CTLL- 2/HIL-4R cell line exhibited susceptible to hlL-4 at a dose as low as 0. 09U /ml and regular dose-response curve. It is , threfore , recommendatory that CTLL-2/HIL-4R cell line could be adopted to accturately assess hlL - 4 bioactivity.更多还原