【摘要】 利用基因工程技术在大肠杆菌表达成功人白细胞介素２（ＩＬ－２）和人血管生成素（Ａｎｇｉｏｇｅｎｉｎ）的融合蛋白（ＩＬ－２－Ａｎｇ）。活性检测证明，它具有ＩＬ－２免疫毒素的作用，在体外可以杀伤ＩＬ－２依赖性ＣＴＬＬ细胞株和经ＣｏｎＡ活化的小鼠脾脏ＩＬ－２Ｒ阳性细胞。在双相混合淋巴细胞培养试验中，ＩＬ－２－Ａｎｇ可抑制淋巴细胞的增殖。在体内实验中，ＩＬ－２－Ａｎｇ注射小鼠可使其抗原活化的脾细胞数量减少。由于ＩＬ－２和Ａｎｇ均为正常人体成分，ＩＬ－２－Ａｎｇ融合分子作为人源化免疫毒素可望具有更好的应用前景。更多还原

【Abstract】 A novel fusion protein namely IL- 2-Ang consisting of interleukin-2 ( IL-2 ) and angiogenin ( Ang) was expressed in E. coli by using genetic engineering technique. Bioassays showed that it could be used as an IL-2 immunotoxin. In vitro ,the IL-2-Ang exhibited cytotoxity effects on the IL-2-dependent cell line CTLL as well as murine IL-2R positive splencytes activated by ConA. In vivo, injections of IL-2-Ang into mice could decrease the splencytes activated by antigen. Since both of the IL- 2 and Ang originated from human beings , the IL-2-Ang could be used as new generation of humanized IL-2 immunotoxin.更多还原