【摘要】 探讨清除循环中毒素后生长因子和残存肾小球的改变。方法采用分子杂交技术和组织病理分析方法在肾次全切除的残存肾大鼠模型上研究腹膜透析（ＰＤ）对转化生长因子β１（ＴＧＦ－β１）ｍＲＮＡ在残存肾中表达的影响和肾小球硬化的变化。分模型组和ＰＤ组，第８周ＰＤ组开始作腹膜透析至第１２周。分别在第７周和１２周各组杀检６只作肾病理检查（ＨＥ．ＰＡＳ染色）和ＴＧＦ－β１ｃＤＮＡ缝隙点样杂交。结果在实验的第７周两组间肾小球硬化指数（ＧＳＩ）及ＴＧＦ－β１ｍＲＮＡ表达均无显著性差异。第十二周，ＰＤ组的ＧＳＩ及ＴＧＦ－β１ｍＲＮＡ表达均显著低于模型组。ＧＳＩ改变程度与ＴＧＦ－β１ｍＲＮＡ表达丰度变化呈明显正相关（ｒ＝０．６５Ｐ＜０．０５）。结论腹膜透析可明显减少ＴＧＦ－β１ｍＲＮＡ的表达并延缓残存肾小球的硬化更多还原

【Abstract】 bjectiveToclarifythechangesofremnantglomeruliandtransforminggrowthfactors-β1(TGF-β)aftertheclearanceofcirculatingtoxins.MethodsSlot-blothybridizationwithcDNAprobeandhistopathologicalanalysiswereusedwithsubtotalnephrectomyratmodeltoevaluatethechangeofglomerularscleriosisandtheeffectofperitonealdialysis(PD)ontheexpressionofTGF-β1mRNAinremnantkidney;RatsweredividedtoPD、modelandcontrolgroup;PDgroupwasrecieveddialysisfor4weeksfromeighthweek;PathologicalandTGF-β1cDNAslot-blothybridizationwereperformedinseventhandtwelfthweekrespectively.ResultsInseventhweek,therewasnosignificantdiferenceofglomerularscleriosisindex(GSI)andTGF-β1mRNAexpressionbetweenPDandmodelgroup;Intewlfthweek,GSIandTGF-β1mRNAexpressionofPDgroupweresignificantlowerthanthatofmodelgroup;TherewasapositivecorrelationbetweenthedegreeofGSIandtheabundanceofTGF-β1mRNAex┐pression.ConclusionPDcanreduceobviouslytheexpressionofTGF-β1mRNAandatenuateglomerularsclerio-sis.更多还原