【摘要】 研究反应性氧代谢产物（ＲＯＭ）在大鼠庆大霉素（ＧＭ）肾毒性中的致病作用及其预防。在腹腔注射庆大霉素的同时，分别给人参总皂甙（ＧＳ）、去铁敏（ＤＦＯ）和丹参（ＳＭ），各组用药均为７天。结果显示各组尿ＮＡＧ酶和血肌酐均比正常组显著增高，且模型组更显著升高。血清和肾皮质匀浆中丙二醛（ＭＤＡ）含量模型组比其他各组显著升高，而各预防组与正常组之间差异不显著；各组血清及肾皮质匀浆中超氧化物歧化酶（ＳＯＤ）活性均比正常组显著降低，而模型组下降更显著；血清和肾皮质匀浆中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性变化与ＳＯＤ变化相类似。光镜发现模型组近曲小管上皮细胞（ＰＴＥＣ）广泛变性与坏死，远曲小管上皮细胞亦有空泡变性，各预防组ＰＴＥＣ仅有局灶至片状的变性，坏死偶见。结果提示ＧＳ、ＤＦＯ和ＳＭ可能通过清除ＲＯＭ而起到预防ＧＭ所致的急性肾衰的作用。更多还原

【Abstract】 Theexperimentwasaimedtostudytheroleofreactiveoxygenmetabolites(ROM)ingentamicinnephrotoxicityinratsandtheprotectiveefectofROMscavengers.MaleWistarratswerealocatedrandomlyinto1.normalgroup;2.modelgroup;3.ginsenoside(GS)gronp;4.desferoxamine(DFO)group;5.salviaemiltiorhiza(SM)group.TheresultshowedurineNAGandScrweresignificantlyhigheringroups2～5thanthatingroup1,andingroup2thanthatinothergroups;serummalondialdehyde(SMDA)andrenalcorticalMDA(RCMDA)lev-elsinmodelgroupweresignificantlyhigherthanthoseingroups1、3、4and5,thediferencesbetwennormalandGS,DFOorSMgroupswerenotsignificant;SODactivityofserumandrenalcortexdecreasedsignificantlyinalother4groupsthanthatinnormalgroup,andinmodelgroupthanthatinGS、DFOorSMgrowps;changesofSGSH-PxandRCGSH-PxactivityweresimilartothoseofSOD.Lightmicroscopyshowedextensivedegenerationandnecrosisinproximaltubuleepithelialcels(PTEC)andvacuolizationindistaltubulesinmodelgroup,butPTECshowedonlyfocaltopatchdegenerationinGS、DFOandSMgroups,necrosiswasseldom.Thedatasug-gestedthatGS、DFOandSMmayplayasoleintheprotectionofGMnephrotoxicityasaROMscavenger.更多还原