【摘要】 目的:了解人体骨骼肌失神经后细胞增殖状态的变化规律,初步探讨可能反映肌肉不可逆改变的指标。方法:42例周围神经损伤术中取材。(1)HE染色:VIDS Ⅲ图象分析系统测肌细胞的截面积。(2)增殖细胞核抗原(PCNA)单克隆抗体免疫组织化学染色统计阳性染色细胞核数。结果:神经损伤后不同时间组肌细胞的截面积差异无统计学意义。结论:人骨骼肌细胞失神经支配后3~4个月时可见PCNA阳性染色核数最多,以后明显减少,各时间组间差异有统计学意义。结论:人骨骼肌细胞失神经支配后3~4个月内其增殖保持高水平,而后明显降低,故神经损伤后应力争早期修复。细胞增殖状态指标是反映肌肉萎缩是否可逆的潜在指标,能帮助我们判断神经修复手术的预后,进而指导临床手术方法的选择。更多还原

【Abstract】 <Abatract>Objective: To investigate the changes of cell proliferation after skeletal muscle denervation in human. Methods: Biopsies were done in 42 cases of peripheral nerve injuries. The cross sectional areas of muscle cells were measured by VIDSIII Image Analysis System in HE stained sections; im-munohistochemical stain (ABC method) with monoclonal antibody against proliferation cell nuclear anti-gen(PCNA) was performed and positive stained nucleus were counted. Results: No statistical difference was found between different time groups after nerve injuries in cross sectional areas of muscle cells. The number of PCNA positive cell nucleus reached maximum at 3-4 months after denervation and then decreased, there was significant difference between different time groups. Conclusions: The proliferation of human skeletal muscle cell remains at high level 3-4 months after denervation and then slows down. This suggests that nerve repair should be performed early. Examination of cell proliferation is possible to indicate if the denervation of cells is irreversible, which is helpful in chosing proper surgery and progno-sing the effect of nerve repair.更多还原