【摘要】 取健康人外周肝素抗凝血,用细胞分离液分离出中性白细胞(PMN)以1×10~6/ml浓度与氟哌利多、咪唑安定、芬太尼、乙咪酯、羟丁酸钠麻醉药物四种不同剂量的稀释液,对照组为生理盐水混匀分别置于24孔培养板内孵育。孵育后10分钟、30分钟、2小时、24小时又分别加等量6～9亿/ml白色葡萄球菌液再孵育45分钟后涂片镜检。计算各种药物、各浓度、各时间的PMN吞噬率。结果表明,五种麻醉药随剂量增大PMN吞噬率逐渐下降,与对照组有显著差异。但与作用时间长短无差异。临床应用以上麻醉药应注意剂量及围术期抗生素及化疗药物应用。更多还原

【Abstract】 The effects of intravenous anesthetics on phagocytosis of human neutrophils were observed in vitro. 1×10~6/ml of neutrophils, isolated from heparinized venous blood of healthy human,was mixed at equivalent volume with normal saline (as control), four different concentrations (clinical doses) of droperidoI, midazolam, fentanyl,etomidate or sodium hydroxybutyrate respectively. All mixtures were incubated in each of four 24-pore culture plates. The equivalent volumes of staphylococci 6～9×10~9/ml were added to these mixtures, 10 mins, 30 mins, 2hs or 24hs after incubation individually. The successive incubations lasted 45 mins,and the neutrophil phagoeytosis rate (NPR) was calculated. The results showed that NPR was correlated negatively with the concentration of each anesthetic mentioned above. but was related to its acting-duration. It is demonstrated that these intravenous anesthetics may reduce the phagoeytosis of neutrophils in a dose-related way,therefore we suggest that antibiotics or antineoplastics should be used in combination with these anesthetics during operation.更多还原