【摘要】 1993～1995年作者在连云港市选近三年曾有脊髓灰质炎病例的乡(镇),采用氯化钠—三氯化铝沉淀法对河塘污水中脊髓灰质炎病毒(PV)循环动态进行研究。发现:在连续二年的各个季节采集的水样中均能分离到致Hep-2细胞病变的病毒(57.95%),经鉴定:21株为PV(23.86%),其中PV Ⅰ 18株、PV Ⅱ 1株、PV Ⅲ 2株,另30株(34.09%)为非脊灰肠道病毒(NPEV)。鉴于我国目前脊灰流行毒株主要为PV Ⅰ,经RT-PCR试验,所分离的PV Ⅰ均为疫苗(Sabin)相关株,初步表明调查地区的外环境的PV已被疫苗相关株所取代。更多还原

【Abstract】 A study on polio virus in pond foul water of town and counties in Lian Yun Gang city which had a reported case of polio. Foul water was examined by 1% NaCl-AlCl₃ precipitated method quarterly during 1993-1995, to observe the cyclic development of the virus. Results showed that the isolation rate of polio virus was 57.95% with 21 strains of 18 PV Ⅰ, 1 PV Ⅱ and 2 PV Ⅲ and 30 strains of NPEV （34.09%）. In view of PV Ⅰ as main epidemic strain in our coun try, and related strains to sabin was confirmed using RT-PCR. The above data suggested that polio virus in the pond water had been replaced by relative strains of sabin.更多还原