【摘要】 本实验通过功能矫治器引导大鼠下颌前伸，采用免疫组织化学的ＡＢＣ法在原位上确定髁突软骨胰岛素（ｉｎｓｕｌｉｎ，Ｉｎｓ．）的分布，探讨其在矫形治疗中的作用及与髁突生长改建之间的关系。结果显示，髁突软骨Ｉｎｓ．分布呈明显的染色梯度，即生发层染色最深，阳性率较高，其次为过渡层，再次是成熟层；实验组髁突中后份，特别是生发层明显增厚，阳性细胞染色深，比例大，其余各层染色亦略深于对照组。本研究表明，髁突软骨中Ｉｎｓ．的分布有一定规律。髁突软骨增生旺盛时，Ｉｎｓ．分布较多，分化活跃时分布则减少。功能矫形治疗可使髁突软骨明显增生，Ｉｎｓ．分布亦随之增加，说明Ｉｎｓ．与髁突软骨增生密切相关。更多还原

【Abstract】 Thepurposeofthisstudyistoevaluateinsulinchangeincondylarcartilageduringfunctionaladvancementofratmandiblebyimmunohistochemicaltechnique(ABCmethod)inordertoprovidefurtherinformationabouttheroleofinsulininorthopedicsandtherelationshipbetweenin-sulinandproliferationofcondylarcartilage.Staininggradientofinsulinisobservedincondylarcarti-lage,withthestaindeepestinthegeriminallayer,medialinthetransitional,leastinthematura-tionallayer.Theposteriorregionofcondylarcartilageintheexperimentalgroupsisshowntohavethickenedsignificantlyascomparedwiththecontrolgrups,especialythegerminallayer.Stained-positivecelsexhibitdeeperstainandhigherpercentage.Thestainintheotherlayersisalsomoreobviousthanthatinthecontrolgroups.Theresultssuggest:Insulinlevelincondylechangeswiththediferentcartilagelayers.Insulindistributionappearshigherwhencondylarchondrocytesprolif-eraterapidlyandlowerwhentheydiferentiateactively.Functionalorthopedicsmaystimulatethegrowthofcondylarcartilageandraisetheinsulinlevel.Thedemonstratesthatinsulincorrelateswithhyperplasiaofconylarchondrocytes.更多还原