【摘要】 利用定量逆转录PCR技术,检测肿瘤转移抑制基因nm23-H1 mRNA,在25例大肠癌及其相应正常粘膜组织中的表达。25例中,22例nm23-H1 mRNA在癌组织的表达,较在相应正常粘膜中的表达高。nm23-H1 mRNA的表达水平与肿瘤大小、分化程度、浆膜浸润及淋巴结转移无显著相关关系(P>0.05)。但有肝转移的大肠癌组织nm23-H1 mRNA的表达较无肝转移者显著减低(P<0.05)。结果表明,nm23-H1基因在抑制大肠癌肝转移方面有重要意义。本方法灵敏度高,特异性强,结果可靠。更多还原

【Abstract】 We studied the expression of tumor metastatic suppressor gene nm23-H1 mRNA in 25 cases of colorectal cancer and their corresponding normal mucosa by using quantitative RT-PCR method. 22/25 cases of colorectal cancer had increased level of nm23-Hl mRNA comparing with their corresponding normal mucosa. There was no significant correlation between expression of nm23-H1 mRNA and tumor size, grade of differenciation and lymph node metastasis(P>0.05).However, nm23-Hl mRNA expression was significantly reduced in tumor with liver metastasis, when compared with tumors without liver metastasis(P<0.05).This observation indicates that nm23-Hl gene may play an important role in inhibiting liver metastasis in colorectal.cancer. This method has high sensitivity and specificity, and is reliable.更多还原