【摘要】 目的 :探讨放射对肿瘤细胞再增殖的影响。材料与方法 :通过不同剂量的放射作用、以BrdUrd/DNA双参数流式细胞术分析放疗后不同时间人鼻咽癌细胞株CNE的增殖动力学变化 ;同时以极限稀释法检测其克隆源性细胞存活比率。结果 :(1 )在 0～ 8Gy剂量范围内 ,CNB细胞群整体Tpot的缩短与其中克隆源性细胞的减少密切相关 ,同时随着剂量增大 ,提示DNA合成增加 ;和 (2 )离体培养中、放射后 48小时内CNB细胞群中克隆源性细胞的增殖状况变化与整体一致。结论 :(1 )放射后CNE细胞的加速再增殖可能与其自身调节有关 ;和 (2 )Tpot可能作为临床预测指标更多还原

【Abstract】 Purpose: Repopulation is among those which causes treatment failure in cancer radiotherapy. The study focuses on a better understanding of radiation-induced repopulation for cancer cells.Materials and Methods: It was measured by BrdUrd/DNA flow cytometric analysis that the kinetic changes after various radiation dose-time effects on human nasophayngeal carcinoma cells. The surviving fraction of the clonogenic cells was also measured by limiting dilution method.Results: (1) the shortening of Tpot of overall cell population after 0～8 Gy of irradiation was closely related to the decrease of the clonogenic cells;(2) an increased DNA synthesis was suggested as a result of the increase of radiation dose and (3) in vitro ,the proliferative status of the clonogenic cells was as same as that of overall cell population 48 hours after irradiation.Conclusion: (1) the accelerated repopulation of CNE cells after irradiation is related to their self-regulation;and (2) Tpot may be used as a predictive value in clinical radiotherapy.更多还原