【摘要】 为了探索人乳头状瘤病毒（HPV）与食管癌发生的关系,对食管鳞癌、癌旁鳞状上皮及乳头状瘤进行组织病理学研究,并用生物素标记的HPV₁₆DNA探针进行原位杂交。结果:癌旁鳞状上皮有凹空细胞等HPV感染的组织形态学改变者为65.2%（15/23）,HPV₁₆₀DNA检出率为56.5%（13/23）;食管鳞癌有凹空样癌细胞者为27.8%（10/36）,HPV₁₆DNA检出率为50.0%（18/36）;6例乳头状瘤均检出HPV₁₆DNA（100.0%）。不同分化程度的食管鳞癌（Ⅰ、Ⅱ、Ⅲ级）HPV₁₆DNA检出率分别为100.0%（6/6）,60.0%（9/15）和20.0%（3/15）。研究结果提示,HPV感染与食管鳞癌发生有关,并与其分化和某些组织学特征有关。更多还原

【Abstract】 Tissues of esophageal squamous cell carcinoma (ESQCC) ,papillo-ma and squamous epithelia adjacent to carcinoma (SEAC) were studied histopathologically and by in situ hybridization with HPV16DNA probe. Of the 23 cases studied 65. 2% (15/23) was found to display koilocytosis and in 56. 5% (13/23) HPV16 DNA was positive in SEAC. Koilocytic cancer cells were observed in 27.8% (10/36) of ESQCC and HPV16DNA was detected in 50. 0% (18/36) of ESQCC;HPV16DNA was detected in all 6 cases of papilloma (100. 0%). The detection rates of HPV16DNA in grade Ⅰ,Ⅱ,Ⅲ ESQCC were 100. 0% (6/6) ,60. 0% (9/ 15) ,and 20. 0% (3/15) respectively. The results of this study indicate that HPV infection is associated with the development,differentiation and some histologic characteristics of ESQCC.更多还原