【摘要】 通过对20例健康国人及肠外营养大鼠应用甘露醇和乳果糖后,测量它们在尿中的浓度比来探讨测量肠粘膜通透性的方法及其结果.研究方法:(1)动物试验:Wistar大鼠20只,行中心静脉插管给予完全肠外营养8天.于肠外营养第3、7天用甘露醇(50mg)和乳果糖(100mg)溶液灌胃,收集24小时尿液,测定尿中甘露醇和乳果糖浓度及其比值;(2)健康志愿者研究:20名健康成人口服甘露醇(5克)和乳果糖(10克)溶液后,收集6小时内的全部尿液,测量尿中的甘露醇和乳果糖浓度及其比值;测量方法采用特殊的电化学高效液相色谱法(HPLCPED).先作出标准曲线,然后用标准曲线公式来求值.研究结果:甘露醇和乳果糖的变异系数分别是2.3%和5.4%,回收率是94.3%～101.9%.大鼠营养支持3天时L/M为0.024.7天时L/M为0.042(通透性相比P<0.05).健康志愿者甘露醇和乳果糖之比(L/M)为0.021±0.0016.结论:特殊的HPLC(HPLCPED)是一种简便、快速和准确测量甘露醇和乳果糖的方法,临床应用较方便.大鼠接受肠外营养后肠粘膜通透性明显增加,测量肠粘膜通透性是了解胃肠道屏障功能的一种间接方法.更多还原

【Abstract】 Purpose:To investigate the method of intestinal permeability measurement in human and rats by using the ratio of mannitol and lactulose in urine. Methods: (1)Rats study: 20 Wistar rats were lavaged with mannitol and lactulose solution. The rats’ 24 hrs urine were collected and the mannitol and lactulose concentration in urine were measured. (2)Study of volunteers:20 healthy male and female were enrolled. They were asked to take 5g mannitol and 10g lactulose, their urine of 6 hrs after taking the mannitol and lactulose were collected and their concentration in urine were measured. The mannitol and lactulose concentration were determined by HPLC with Pulsed Electrochemical Detection (HPLC-PED). A standard curve was created. Results: The CV of mannitol and lactulose were 2.3% and 5.4% respectively. The recovery rate was 94. 3%-101. 9%. After three days of parenteral nutrition, the L/M was 0. 024. The L/M became to 0. 042 after 7 days of parenteral nutrition (P < 0. 05). The ratio of Lactulos/ Mannitol (L/M) of volunteers was 0. 021±0. 0016; Conclusion: The HPLC-PED was a convenient, fast and accurate method for measurement of mannitol and lactulose which was applicable in clinic. The rats’ in testinal permeability increased after receiving parenteral nutrition. Determination of the intestinal permeability might be a way of evaluating bowel barrier function.更多还原