【摘要】 人早幼粒白血病ＨＬ６０细胞及其亚系中ｍｄｒ１基因和癌基因表达的关系周卫东，张鸿卿，方敏，薛绍白（北京师范大学生物系，北京１００８７５，中国）关键词癌基因；多种抗药性；流式细胞计量术；急性早幼粒白血病目的：研究人早幼粒白血病ＨＬ６０细胞系及其分别抗Ｈ...更多还原

【Abstract】 IM: To study the relationship between expression of oncogenes and multiple drug resistant (MDR) phenotype. METHODS: The drug resistant level of HL60 cell line and its sublines were determined with flow cytometry. RNA Dot blot hybridization was used to identify the expression of oncogenes and mdr 1 gene. RESULTS: The expression of mdr 1 gene was in the opposite relation with c myc expression, but in the positive relation with c H ras gene expression in the multiple drug resistant cell lines. In non MDR cell line HL60/RA, the expression levels of mdr 1, c myc , and c H ras were the same as HL60 parental cells. CONCLUSION: Multiple drug resistance is related to not only mdr 1 expression, but also some oncogenes expression level.更多还原