【摘要】 应用细胞培养技术，分子杂交的ＮｏｒｔｈｅｒｎＢｌｏｔ方法和图像细胞分析技术（ＩＣＭ）研究了β－胡萝卜素对体外培养的人乳腺癌细胞株ＢＣａｐ一３７的生物学作用。细胞生长曲线的结果显示，两个实验组（５×１０（－５）ｍｏｌ／Ｌ，１×１０（－４）ｍｏｌ／Ｌ）对细胞的生长有显著抑制作用并伴有明显的细胞形态学特征的改变。抑制率分别为３３．３３％和２８．２５％（Ｐ＜０．０１，Ｐ＜０．０５）。血浆生理浓度组（１×１０（－５）ｍｏｌ／Ｌ）对细胞的生长没有抑制作用（Ｐ＞０．０５）。基因表达的研究显示，β－胡萝卜素（１×１０（－５）ｍｏｌ／Ｌ，１×１０（－４）ｍｏｌ／Ｌ）对癌基因Ｃ一ｅｒｂＢ２ｍＲＮＡ的表达没有抑制作用，１×１０（－４）ｍｏｌ／Ｌ浓度组对癌相关基因ＥＧＦＲｍＲＮＡ的表达有抑制作用，应用ＩＣＭ技术测定四项指标，即ＤＮＡ指数（ＤＩ值），Ｓ期百分比（Ｓ％），总ＤＮＡ含量以及细胞核面积。结果显示，两实验组（１×１０（－５）ｍｏｌ／Ｌ，１×１０（－４）ｍｏｌ／Ｌ）ＤＩ值为１．８１和１．７４，反映细胞呈异倍体水平，为恶性细胞的特征。两组的Ｓ期百分比为３３．７０％和３０．３９％，核面积为１４３．０μＭ２和１２０．９０μＭ２，均低?更多还原

【Abstract】 iological effect of beta一carotene on Chinese breast cancer cell line- BCap-37 was studied by metliods of cell culture, Northern Blot and Image Cytoinetry(ICM).Cell growth ctirves showed that beta-caroteiic(5×10(-5)mol /L,1 × 10(-5)mol/L)inhibited significantly the cell growth,and the inhi-bitory rates were 33.33%and 28.25%respectively(P<0.01,P<0.05).Theseeffects were eaccompanied by markedly cell morphological changes.Bloodphysiological concentration(1×10(-5)mol/L)didn’t inhibit sikynificantly thecell growth (P>0.05).Tlie results of gene expression demonstrated that beta,carotene(1×10(-5)mo/L,1 ×10(-4)mol/L)didn’t inhibit C- erbB2 mRNAexpression,while the concentration of 1×10(-4) mol/L inhibited EGFR mRNAexpressiOn,Four iteins,DNAindex(DI),S- phase percentage (S%), totalDNA conteIlt and nueleus area,were tested by Image Cytonletry (ICM).DI1n two groups(1×10(-5) mol/L,1×10(-4)mol/L)were 1.84 and 1.74, which showedthe feature of malignant tumour cells.S- phase percentages were 33.70%and30.39%total DNA contents were 18.23 pg and 16.69pg,and nucleus areaswere 143.00μM2 and 120.90μM2.They were significantly lower than the con-trol group(P<0.05, P<0.01).These results of ICM stggested thdat beta-carotent inhibited singificantly BCap- 37 cell proliferation.更多还原