【摘要】 研究了金龟子绿僵茵（Ｍｅｔａｒｈｉｚｉｕｍａｎｉｓｏｐｌｉａｅ）在土壤中的发育过程、数量消长及控制桃小食心虫的潜力．结果表明，接种６２~８２ｄ后所有处理的ＣＦＵ均降低８０％以上，１２１~２３４ｄ后降低９９％以上．在灭菌和不灭菌土壤中，分生孢子初始接种量的半衰期分别为２８．５ｄ和２３．８ｄ，干菌丝粉分别为２６．９ｄ和１９．６ｄ．在土壤接种后１００ｄ，将桃小食心虫冬茧接入土壤，冬茧被侵染产孢后补偿了带菌量的下降，２７８ｄＣＦＵ比不接冬茧土壤第２７１ｄ的带菌量大１０００倍．在土壤接种后，不同时间接入土壤的冬茧死亡率直到第１３１ｄ还保持在９０％以上，但到第２３７ｄ降至９．３％．未用绿僵菌处理的土壤中接入的桃小食心虫冬茧无死亡发生．小区试验中，在１５、２２．５、３０和３７．５ｋｇ·ｈｍ（－２）分生抱孢制剂剂量下死亡率达９７．０~１００％，而蛀果率仅为２．７~５．０％．在陕北进行了大田试验面积达６７０ｈｍ２，剂量为２２．５ｋｇ·ｈｍ（－２）分生孢子制剂．蛀果率降至２．４％，未处理果园则高达３０％．更多还原

【Abstract】 The development process and quantitative dynamics of Metarhizium anisopliae in soil and its potential in controlling Carposina nipponensis are studied.The results show that the number of its colony forming units CFU is declined by more than 80% in all treatments after 62￣82 days,and by more than 99% after 121￣234 days.The half-life of initial inoculum are 28.5 and 23.8 days for conidia and 26.9 and 19.6 days for mycelia in sterile and non-sterile soil,respectively.With C.nipponensis cocoons containing larvae introduced 100 days after soil inoculation,their infection is compensated for the decline,and fungal recovery is 1000 times larger in 278 days than in 271 days after inoculation without cocoons.The mortality of C.nipponensis larvae introduced at different time after soil inoculation is remained by more than 90% by day 131,and is declined to 9.3% by day 237.C.nipponensis is not infected in untreated soil. In plot trials, 97￣100% of larvae are killed with doses of 15,22.5,30 and 37.5 kg conidial preparation per hectare,and the rates of bored fruit are 2.7￣5.0%.Pilot tests are conducted with an application rate of 22.5 kg·hm(-2)in 670 hm2 of north Shaanxi.The rate of bored fruit is reduced from 30% for untreated orchards to 2. 4% for treated ones.更多还原