【摘要】 利用Ｎｏｒｔｈｅｒｎ印迹杂交及逆转录聚合酶链式反应（ＲＴ－ＰＣＲ）技术研究了几种传代培养细胞和成人肝组织及人胚胎肝组织细胞色素Ｐ４５０１Ａ１（１Ａ１）基础表达水平并观察了几种化学诱导剂对人羊膜细胞ＦＬ系和原代胚肝细胞该基因ｍＲＮＡ水平的诱导．结果显示：３－甲基胆蒽，苯巴比妥，β－萘黄酮在不同程度上能高诱导ＦＬ细胞１Ａ１ｍＲＮＡ表达．ＲＴ－ＰＣＲ结果显示ＣＨＬ细胞及Ｖ７９细胞存在基础表达，但Ｎｏｒｔｈｅｒｎ杂交结果提示其表达量极低．成人肝脏中１Ａ１亦存在极低的表达水平，而人胚胎肝脏不能用高灵敏度的ＲＴ－ＰＣＲ检测到痕迹表达，提示胚胎发育时期１Ａ１基因处于完全关闭状态．更多还原

【Abstract】 Induction of cytochrome P450 1A1 (Cyt P450 1A1) gene expression in liver tissues and in culture cell lines in vitro by chemical agents was studied. Our data showed that 3-methylcholanthrene, β-naphthoflavon and phenobarbital could highly induce the level of Cyt P450 1A1 mRNA in FL cell, but not in liver tissue cells. Cyt P450 1A1 mRNA was detectable in Chinese hamster cell line CHL and V79 by RT-PCR, but the Northern hybridization analysis indicated that the levels of expression was very low. RT-PCR could detect Cyt P450 1A1 mRNA expression in human adult liver tissues. However, it could not detect in embryo liver tissues in various developmental periods. This suggests that Cyt P450 1A1 gene expression is switched in the developmental periods of embryo liver.更多还原