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链亲和素-胶体金原位杂交检测急性白血病的多药耐药基因表达

Expression of Multidrug Resistance Gene in Acute Leukemia by In Situ Hybridization with Streptavidin - Gold

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【作者】 杜跃斌艾辉胜王桂林汲言山胡美茹程海云黄云中楼方定

【Author】 DU Yue - Bin AI Hui - Sheng WANG Gui - Lin JI Yan - Shan HU Mei - RuCHENG Hai - Yun HUANG Yun - Zhong LOU Fang - Ding(Beijing Bei Tai Ping Road Hospital Beijing 100039)

【机构】 北京北太平路医院北京基础医学研究所长城医院血液科 北京 100039北京 100039北京 100850北京 100853

【摘要】 用链亲和素-胶体金原位杂交(ISH-SAG)检测了52例急性白血病和4例正常骨髓细胞的多药耐药基因(MDR1)表达。全部52例急性白血病中25例(48.1%)MDR1呈阳性表达,其中初治组MDR1阳性12例(43%),复发难治组MDR1阳性11例(78.6%),两组相差显著P<0.05,完全缓解期MDR1阳性2例(20%)。疗效分析发现MDR1表达与临床缓解率密切相关,MDR1阳性组完全缓解率(52.1%)明显低于MDR1阴性组(88.2%),P<0.05。本研究还同时用免疫细胞化学法检测了P-糖蛋白(Pgp)的表达,两者结果的一致率为84.6%(40/52)。初步结果表明:对检测MDR1检测简便、敏感和特异,为多药耐药的临床研究提供了一个新方法。用ISH-SAG检测MDR1,可预测白血病对化疗的敏感性及预后,并为制定化疗方案提供依据。

【Abstract】 Expression of MDRl mRN A in marrow celis from 52 patients with acute leukemia (acute myeloid leukemia 37, acute lymphoblastic leukemia 15) by in situ hybridization with streptavidin - gold (ISH- SAG) was studied. On the 52 cases of acute leukemia, MDRl positive rate was 43% (12/28) in the initial diagnosed patients, 79%(11/14) in relapse or resistant patients (P<0.05), 20% (2/10) in complete remission patients. Complete remission rate was 52% (12/23) in MDRl positive cases and 88% (15/17) in MDRl negative cases (P<0.05). Simultaneously, expression of multidrug resistance gene associated P - glycoprotein(Pgp) in marrows celis was studied from 52 patients by means of a Pgp- directed monoclonal antibady (JSB- 1) and immunocytochemistry. Pgp positive rate was 29% (8/ 28) in the initial diagnosed patients and 64% (9/13) in relapse or resistant patients (P<0.05). Coin-cidence between expression of MDRl and Pgp was 85% (44/52). These data show that the determina-tion of MDRl expression in acute leukemia could provide useful information for designing chemothera-peutic regimen and prediction for prognosis. ISH - SAG is a relative simple, specific and sensitive method for detection of MDRl gene expression, also may simultaneously observe the morphology of cells.

  • 【文献出处】 实验血液学杂志 ,Journal of Experimental Hematology , 编辑部邮箱 ,1996年03期
  • 【分类号】R733.71
  • 【被引频次】10
  • 【下载频次】43
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