【摘要】 用链亲和素-胶体金原位杂交(ISH-SAG)检测了52例急性白血病和4例正常骨髓细胞的多药耐药基因(MDR1)表达。全部52例急性白血病中25例(48.1％)MDR1呈阳性表达,其中初治组MDR1阳性12例(43％),复发难治组MDR1阳性11例(78.6％),两组相差显著P<0.05,完全缓解期MDR1阳性2例(20％)。疗效分析发现MDR1表达与临床缓解率密切相关,MDR1阳性组完全缓解率(52.1％)明显低于MDR1阴性组(88.2％),P<0.05。本研究还同时用免疫细胞化学法检测了P-糖蛋白(Pgp)的表达,两者结果的一致率为84.6％(40/52)。初步结果表明:对检测MDR1检测简便、敏感和特异,为多药耐药的临床研究提供了一个新方法。用ISH-SAG检测MDR1,可预测白血病对化疗的敏感性及预后,并为制定化疗方案提供依据。更多还原

【Abstract】 Expression of MDRl mRN A in marrow celis from 52 patients with acute leukemia (acute myeloid leukemia 37, acute lymphoblastic leukemia 15) by in situ hybridization with streptavidin - gold (ISH- SAG) was studied. On the 52 cases of acute leukemia, MDRl positive rate was 43% (12/28) in the initial diagnosed patients, 79%(11/14) in relapse or resistant patients (P<0.05), 20% (2/10) in complete remission patients. Complete remission rate was 52% (12/23) in MDRl positive cases and 88% (15/17) in MDRl negative cases (P<0.05). Simultaneously, expression of multidrug resistance gene associated P - glycoprotein(Pgp) in marrows celis was studied from 52 patients by means of a Pgp- directed monoclonal antibady (JSB- 1) and immunocytochemistry. Pgp positive rate was 29% (8/ 28) in the initial diagnosed patients and 64% (9/13) in relapse or resistant patients (P<0.05). Coin-cidence between expression of MDRl and Pgp was 85% (44/52). These data show that the determina-tion of MDRl expression in acute leukemia could provide useful information for designing chemothera-peutic regimen and prediction for prognosis. ISH - SAG is a relative simple, specific and sensitive method for detection of MDRl gene expression, also may simultaneously observe the morphology of cells.更多还原