【摘要】 现场观察证明,钉螺在北纬33°15′以北地区生殖腺发育受到抑制,生殖力下降。为探索其机制,本文采用组织学、组织化学、酶组织化学与透射电镜技术,对放养在山东济宁、江苏徐州及镇江钉螺的生殖腺作了观察。结果显示:北移钉螺雌、雄生殖腺均萎缩,被空泡样结构取代;糖原、DNA和组蛋白含量减少;CEO、5′-NT、LDH和G-6-Pase活性降低;睾丸内SDH活性增高;精子数量减少,并呈不同程度的变性等。观察提示,北移钉螺生殖腺代谢障碍是导致腺体萎缩。生殖机能减退的重要原因。更多还原

【Abstract】 Our field experiments have proven that when Oncomelania snails have been fed in north-ward 33°15’ north latitude regions the growth of their gonads would be inhibited and they would have lower reproductive rates. In order to further evaluate the mechanism, the gonads of Oncomelania snails fed in Jining, Shandong Province (35°23’ north latitude), Xuzhou, Jiangsu Province (34°21’ north latitude) and Zhengjiang,Jiangsu Province (32°10’ north lati-tude ,control group) were observed in histology, histochemistry,enzyme-histochemistry and ultrastructure. The results showed the testes and the ovary of Oncomelania snails fed in Jin-ing and Xuzhou were atrophied ;the contents of glycogen,DNA and histone and the activities of cytochrome oxidase (CCO) ,5’-Nucleotidase(5’-NT) ,LDH and G-6-Pase were inhibited in the testes and ovary of these snails. But the SDH activity was elevated in the testes. The ultrastructural observation showed the number of sperm had reduced, the sperms were dena-tured in vary degrees in testes of the snail in Jining and Xuzhou.更多还原