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荧光分光光度法测定氧氟沙星片剂的含量
etermination of ofloxacin in tabletsby fluorospoectrophotometry
【摘要】 用荧光分光光度法测定氧氟沙星片剂的含量。选用0.05mol/L盐酸为溶剂,在Ex=293nm、Em=507nm处测定荧光强度。在0.10~0.35ug/ml范围内,荧光强度与浓度呈线性关系,回归方程为C=0.0736F—0.0069(r=0.9998)。平均回收率为99.53%,RSD为0.89%(n=8)。本法灵敏度高,操作简便,结果准确。
【Abstract】 This article reported the assay of ofloxacin tablets by fluorospectrophotometry. Hy-drochloride acid solution was selected as solvent and Ex293nm and Em507nm were set. There was agood correlation between fluoresent value and concentration ranging from 0. 10μg/ml to 0. 35μg/ml.The regression equation was C= 0. 0736F- 0. 0069 (r= 0. 9998). The average recovery was 99.53 %, RSD= 0. 89 % (n= 8). This method is sample, sensitive and accurate.
- 【文献出处】 西北药学杂志 ,NORTHWEST PHARMACEUTCAL JOURNAL , 编辑部邮箱 ,1996年03期
- 【分类号】R927.2
- 【被引频次】3
- 【下载频次】159