【摘要】 采用猫肾传代细胞FK增殖华东地区分离的貂传染性肠炎病毒（mink infectious enteritis virus,MEV-S₁）能产生明显的细胞病变。浓缩的病毒液经Sepharose-4B柱层析提纯处理后置电镜下观察,可见典型的病毒粒子。经蛋白酶K、SDS裂解病毒,用苯酚-氯仿法抽提病毒核酸,二苯胺试验和酶消化试验表明该病毒核酸为DNA类型。吖啶橙染色试验表明该DNA为单链。甲酰胺法进行核酸分子展层,病毒核酸呈线状,长度为1.5～2.0/μm。更多还原

【Abstract】 A strain of mink infectious enteritis virus that was isolated from the east district of China can be bred on FK cell. Concentrated viruses were purified by using Sepharose-4B chromatography. The size of the virus was about 20 - 22nm by electron microscope. Viral nucleic acid was extracted from pure virus by using SDS-proteiase K-Phenol. Tests with diphenylamine, acridine orange and the curve of thermal dentuation, etc. showed that the virus had a single-stranded DNA. The molecular weight of the ssDNA was from 1.5 ×106 to 2.0 ×106d determined by length of the virul nucleic acid.更多还原