【摘要】 研究了一株芽孢杆菌（ＢａｃｉｌｌｕｓＳｐ．）产生脲酶的条件及脲酶的提取。结果表明，芽孢杆菌适宜的生长及产酶温度为３５～３７℃，产酶受底物──尿素的诱导。静止培养有利于产酶，Ｎｉ２＋对产酶没有影响。脲酶粗提物经硫酸铰盐析、热处理、ＳｅｐｈａｄｅｘＧ—２００及ＤＥＡＥ－纤维素柱层析等提取步骤脲酶比活力提高了近７０倍，回收率１５．５％。更多还原

【Abstract】 The paper describes the experimental results of production and extraction of ureasefrom Bacillus sp The results showed that the strain was suitable for temperature of growth and ureaseproduction at 35-37℃, the enzyme production was induced by its substrate-urea, static culturedoes good to enzyme yield,but Ni2+ addition has no effect on it.After some extraction steps such asammonium sulfate fractionation, heat treatment and column chromatography on Sephadex G-200 andDEAE-cellulose,enzyme specific activity was increased about 70 times compared with initial product,the rate if recovery was 15.5%.更多还原