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抗人精子单克隆抗体的制备及特性研究
PREPARATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES TO HUMAN SPERM
【摘要】 用杂交瘤技术制备了两株分泌抗人精子单克隆抗体的细胞株,经酶联免疫吸附试验证明两株细胞株所分泌的单克隆抗体均能与人精子产生特异反应,并有明显的精子凝集和制动作用
【Abstract】 Two hybrid cell lines that secreted monoclonal antibodies (McAbs)to human sperm were obtained by using hybridoma technique.All of the two McAbs showed positive reaction in the enzyme linked immunosorbent assay (ELISA),and could significantly agglutinated and immobilize the human sperm.
【关键词】 抗人精子单克隆抗体;
杂交瘤技术;
凝集;
制动;
【Key words】 McAbs to human sperm; hybridoma technique; agglutinate; immobilize;
【Key words】 McAbs to human sperm; hybridoma technique; agglutinate; immobilize;
- 【文献出处】 天津医科大学学报 ,JOURNAL OF TIANJIN MEDICAL UNIVERSITY , 编辑部邮箱 ,1996年04期
- 【分类号】Q503
- 【下载频次】22