【摘要】 采用直接注射外源ＤＮＡ－脂质体复合物的方法分别在荷瘤裸鼠人肠癌组织中转染外源正义ｐ５３ｃＤＮＡ和反义ｐ５３ｃＤＮＡ的重组表达质粒及非整合型哺乳动物表达载体ｐＲＥＰ９，并以注射等量生理盐水为对照，然后应用链菌素亲生物蛋白－过氧化酶免疫组化法检测肿瘤组织中突变型ｐ５３蛋白的表达。结果表明：体外培养ＳＷ１１１６细胞中约有５０％为突变型ｐ５３蛋白阳性；转染反义ｐ５３ｃＤＮＡ表达质粒组的裸鼠移植瘤细胞ｐ５３蛋白阳性率为２５％，而注射生理盐水、转染ｐＲＥＰ９及正义ｐ５３ｃＤＮＡ表达质粒组阳性率分别为６６．７％，７７．８％，６６．７％；前者与后三者之间有显著住差异（Ｐ＜０．０１，Ｘ［２］检验），但各组间肿瘤大小并无显著性差异。据此认为，肿瘤体内直接转染外源反义ｐ５３基因能封闭肠癌ＳＷ１１１６细胞中突变型ｐ５３基因的表达。更多还原

【Abstract】 Exogenous recombinant expression plasmid of sense p53 cDNA, antisenes p53 cDNA and the expression vector pREP9 were directly transferred into colon adenocarcinoma cells (SW1116) in nude mice respectively by injectng DNA-liposome complex into the tumor body, while control group was injected with tormal saline. Then, the streptin-biotin-peroxidase complex method was performed to detect the expression of p53 gene immunohistochemically in the transfected cells by using the anti-mutant p53 protein as the first antibody. The results demonstrated that half of SW116 cells cultured in vitro contained mutant p53 protein. And positive rates of mutant p53 protein on the sections of transplanted tumor in nude mice treated with antisense p53 cDNA expression plasmid transfectiom,normal saline injection,pREP9.sense p53 cDNA plasmid transfection were 25%,66. 7%,77. 8%,66.7% respectively. There existed a significant difference between the first group and others, and no difference among the latter three groups. While no significant difference was found between the tumor sizes among these four groups. So,a conclusion was drown that expression of exogenous antisense p53 gene could block the synthesis of mutant p53 protein in SW1116 cells.更多还原