节点文献
人活化素结合蛋白的分离及纯化
Separation and Purification of Human Follistatin
【摘要】 为了进一步研究人活化素结合蛋白的免疫生物学活性,从人卵泡液中提取了活化素结合蛋白,采用亲和层析及高效液相层析法,最终获得35KDa,SDS-PAGE显示为一条带的人活化素结合蛋白,经免疫活性测定、激活素结合试验以及FSH分泌抑制试验证实,所纯化的人活化素结合蛋白为具有生物学活性的天然活化素结合蛋白,可供进一步的免疫生物学研究用。
【Abstract】 In order to study on the blological activity of follistatin,it was extracted from human follicular fluul and purified by affinity and high performance liquid chromatographies.SDS-PAGE profile showed that the final product was a single band with a molecular weight of 35KDa. Immunological activity detection,activin binding test and FSH secretion inhibition test proved that the final product was a natural biologically-active follistatin and further study on its immunobiology is needed.
- 【文献出处】 中国生物制品学杂志 ,Chinese Journal of Biologicals , 编辑部邮箱 ,1996年04期
- 【分类号】R341
- 【被引频次】4
- 【下载频次】40