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嗜热芽孢杆菌XJT9503高温中性蛋白酶的研究──Ⅰ.XJT9503菌的特性
Study on the Thermaturic Neutral Proteinase of thed Thermophilic Bacillus XJT9503 ──Ⅰ. The Optimus Conditions for Producing Proteinase and the Charactistic of XJT9503
【摘要】 从新疆吐鲁番盆地土壤里分离到一株嗜热芽孢杆菌,代号XJT9503.能产生胞外高温中性蛋白酶,该菌在旋转式摇床上110r/min,45℃下培养60小时,发酵液产酶为5600单位/ml,但在45℃静止培养产酶可达7400单位/ml,Ca2+、Mg2+对产酶有明显促进作用,Cu2+等抑制产酶。
【Abstract】 The thermophilic bacillus XJT9503 which was isolated from Xinjiang Turpan basin can produce exocellular thermoduric meutral protainase. When cultured on shaking apparatus. 110r/min, 45℃, 60 hours, the production of the proteinase is 5600μ/ml. When cultured still, it can reach 7400μ/ml. Ca2+ .Mg2+ can improve the productioll. Cu2+ call curve the producing obility.
【关键词】 芽孢杆菌;
高温中性蛋白酶;
酶活;
【Key words】 Bacillus; Exocellular thermoduric neutral proteinase; PrbdUction of the proteinase;
【Key words】 Bacillus; Exocellular thermoduric neutral proteinase; PrbdUction of the proteinase;
- 【文献出处】 生物技术 ,BIOTECHNOLOGY , 编辑部邮箱 ,1996年03期
- 【分类号】Q814
- 【被引频次】9
- 【下载频次】109