【摘要】 观察了亚硒酸钠（Ｎａ２ＳｅＯ３）在体外作用于大鼠晶状体上皮细胞（ＲＬＥｃｅｌｌｓ）而造成的ＤＮＡ单链断裂（ｓｉｎｇｌｅｓｔｒａｎｄｂｒｅａｋｓ，ＳＳＢ），并对其ＤＮＡ损伤、修复动力学做了初步研究．发现ＳＳＢ严重程度与亚硒酸钠的浓度呈线性相关，其ＳＳＢ重接修复约在３０～６０ｍｉｎ内完成．还作了有关非程序ＤＮＡ合成（ＵＤＳ）的检测，发现与ＳＳＢ相比，ＵＤＳ发生迟且持续时间更长，提示Ｎａ２ＳｅＯ３可能在体外对大鼠晶状体上皮细胞除造成ＳＳＢ以外，还可能造成其它种类的ＤＮＡ损伤．更多还原

【Abstract】 DNA damage and its repair induced by selenite were observed in cultured rat lens epithelial cells (RLE cells). First, DNA single strand breaks(SSB) were detected in the alkalined eluate of rat lens epithelial cells. Then, the religation of the DNA SSB was observed. Lastly,the unscheduled DNA synthesis (UDS) was detected under the same condition. The results suggested:(1) Positive correlation was observed between SSB and the concentrations of selenite.(2) The repair of SSB completed in about 30-60 minutes. (3) The results of UDS, suggested that there may be other kinds of DNA damage induced by selenite in RLE cells.更多还原