【摘要】 本工作对低频（２Ｈｚ）和高频（１００Ｈｚ）电针引起大鼠中枢Ｆｏｓ／Ｊｕｎ表达和三类阿片肽基因表达进行了详细的比较研究，并用针对ｃ－ｆｏｓ／ｃ－ｊｕｎ的反义寡核苷酸（ＯＤＮｓ）对电针引起的Ｆｏｓ／Ｊｕｎ表达进行选择性阻断，然后观察阿片肽基因表达是否受到影响，以确定Ｆｏｓ／Ｊｕｎ复合物在电针引起阿片肽基因表达中的作用。主要结果如下：（１）２Ｈｚ和１００Ｈｚ电针引起脑内不同部位的Ｆｏｓ／Ｊｕｎ表达；（２）２Ｈｚ电针使前脑啡肽原（ＰＰＥ）ｍＲＮＡ表达大幅度增高，１００Ｈｚ电针也能增加ＰＰＥｍＲＮＡ的转录，但不如２Ｈｚ电针的作用显著；但１００Ｈｚ电针能使某些脑区的前强啡肽原（ＰＰＤ）基因转录加速，而２Ｈｚ电针没有这一作用；（３）用ｃ－ｆｏｓ／ｃ－ｊｕｎ反义ＯＤＮｓ特异地阻断Ｆｏｓ／Ｊｕｎ表达后，电针引起的ＰＰＤ转录加速被明显阻断，而ＰＰＥ表达不受影响。提示Ｆｏｓ／Ｊｕｎ是电针引起ＰＰＤ（而非ＰＰＥ）基因表达的转录因子。更多还原

【Abstract】 The present work was designed(a)to study comparatively the effect of 2Hz and 100Hz electroacupuncture(EA)on the expression of oncogene c-fos/c-jun and three opioid(preproenkephalin-PPE;preprodynorphin-PPD;proopiomelanocortin-POMC)genes in the rat brain;(b)to clarify the role of Fos/Jun(AP-1)on opioid genes expression induced by EA stimulation through specific blockade of EA-induced Fos/Jun expression using antisense oligodeoxynucleotides(ODNs)of c-fos/c-jun.The results were:(a)2Hz and 100Hz EA induced differential Fos expression in different brain areas;(b)EA of both frequencies accelerated PPE gene transcription,but 2Hz EA was more effective than 100Hz EA;(c)PPD expression was accelerated by 100Hz EA,but not by 2Hz EA;(d)The blockade of Fos/Jun expression by c-fos/c-jun antisense ODNs prevented EA from accelerating PPD but not PPE mRNA expression.更多还原