【摘要】 研究α２－巨球蛋白（α２Ｍ）对体外培养黑色素瘤细胞增殖、侵袭能力和组织蛋白酶Ｄ活力的影响。采用镜下计数，检测侵袭粘附在内皮细胞上３Ｈ－ＴｄＲ标记瘤细胞的放射性和比色测定组织蛋白酶Ｄ活性。结果：α２Ｍ可抑制瘤细胞生长、集落形成、侵袭能力和组织蛋白酶Ｄ活力。结论：α２Ｍ具有明显的抗肿瘤细胞增殖及转移能力，值得进一步研究以期应用于临床。更多还原

【Abstract】 PURPOSE To study the effects of α2-macroglobulin on proliferation and invasion of melanoma cell line in vitro. METHODS (l)Melanoma cell lines were maintained in RPMI 1640 culture medium supplemented with 15 % fetal calf serum, glutamine and HEPES. (2)To assess whether α2-macroglobulin possess direct cell growth and colone formation inhibitory effects on melanoma cells, cells were seeded in culture media in the presence of different concentration of this agent. At the end of the incubation period tumor cells were harvested and counted with microscope. (3) The tumor cell invasion model which contains the culture of endothelial cells, radio-labelled tumor cells in vitro was used to do the research about the invasion. (4) Cathepsin D activity in harvested melanoma cells were determined by spectrophotometric assays. RESULTS Experiment showed that the melanoma cell growth, clone formation, invasive potential and cathepsin D activity were significantly inhibited fallowing the addition of α2-macroglobulin. CONCLUSIONS The antineoplastic effect of α2-macroglobulin is worthy to be further investigated.更多还原