【摘要】 寡聚脱氧核苷酸ｄ（ＣＣＧＴＡＣＧＧ）质子共振谱线归属和溶液物象表征王萍，石根斌，宋国强，陈凯先，嵇汝运（中国科学院上海药物研究所，２０００３１）关键词寡聚脱氧核苷酸；２ＤＮＭＲ；溶液构象石蒜内铵是一种新型ＤＮＡ嵌合剂，它可以显著改变ＤＮＡ螺旋的构象。...更多还原

【Abstract】 Lycobetaine Prepared from lycorine is a new anticancer agent.The experimental and quantum Pharmacological studies revealed that lycobetaine can interact with DNA by intercalation,preferentially into GO base pairs.In order to provide detailed interaction model of lycobetaine-DNA,a self-complementary octanucleotide d(CCGTACGG)was designed and synthesized by using new HELP(high efficieney liquid phase)According to its nature,the sample was prepared to the desired final concentration by adding salt and buffer solutions.Two-dimensional 1H-1H COSY and NOESY spectra in 99.8%D2O and 95% H2O were recorded for the duplex,and the NMR techniques of presaturation and WATERGATE were applied to water suppression.Protons of every spin system were identified by their scalar couplings,then through their spacial couplings all protons in the molecule were assigned except the poorly resolved H5’and H5"resonanced. The chemical shifts of exchangeable protons and NOE intensities of nonexchangeable protons indicate qualitatively that the d(CCGTACGG)helix is right-handed B-DNA in aqueous solution.更多还原