【摘要】 体外重组３Ｈ－ＣＥ－无ＡｐｏＥ－ＨＤＬ２（ｒＨＤＬ２）保持天然ＨＤＬ２生物活性。大鼠肝窦状隙细胞与ｒＨＤＬ２３７℃培养３ｈ（正常组）；细胞内吞ｃｐｍ为９９５±１４７（ｘ±Ｓ．Ｄ．，ｎ＝２）。细胞进一步９７℃培养２ｈ．释放三氯醋酸（ＴＣＡ）沉淀和ＴＣＡ上清液中ｃｐｍ为７８±３２和１２±９。Ｎ－乙酰咪唑修饰组为３３９±６２、１９±１１和９±５。肝素处理组为５４２±７８、３４±１４和９±８。实验结果提示：（１）肝窦状隙细胞通过ＨＤＬ受体内吞ＨＤＬ２摄取ＨＤＬ２－ＣＥ，并通过逆向胞饮将ＨＤＬ３排出体外．（２）肝性脂酶（ＨＬ）直接介导细胞选择性摄取ＨＤＬ２－ＣＥ，导致ＨＤＬ３生成．（３）ＨＬ和ＨＤＬ受体在介导细胞选择性摄取ＨＤＬ２－ＣＥ中具有协同作用。更多还原

【Abstract】 The recombined 3H-CE-HDL2(rHDL2)keeps the biological activities of the native HDL2.After rat hepatic sinusoidal cells were incubated with rHDL2 at 37℃ for 3h(normal group),the cell-endocytic cpm was 995±147(x±SD, n=2).After the cells were further incubated for 2h, the cell-released TCA-precipitable cpm and the TCA-supernatant cpm were 78±32 and 12±9 respectively. These valued were 339±62, 19±11 and 9±5 respectively in the acetylimidazole-modified group,and 542±78,34±14 and 9±8 respectively in the heparin-pretreated group.Our results suggestsd that:(1)Rat hepatic sinusoidal cells internalize HDL2 and take up HDL2-CE by its HDL receptor,and HDL3 was secreted ont of the cells by retroendocytosis.(2)Hepatic lipase(HL)induces directly the selective uptake of HDL2-CE by the cells.(3)There is cooperation between the HDL receptor andHL in the selective uptake of HDL2-CE by the cells.更多还原