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人参和西洋参蛋白电泳的鉴别研究

IMPROVED HIGH PRESSURE LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF PAEONIFLORIN IN SEDATIVE HONEY PILL NO. 2 AND IN RADIX PAEOHIAE RUBRA

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【作者】 许欣荣; 赵华英; 霍德兰; 陈永林; 路晓钦;

【Author】 Guo Ruichen; Chu Jianshe; Li Chaowu ; et al(Affiliated Hospital of Shandong Medical University , Centre Hospitaal of Qilu Petrochemical Corp.)

【机构】 山东医科大学中草药学教研室;

【摘要】 人参和西洋参蛋白电泳的鉴别研究许欣荣赵华英霍德兰陈永林路晓钦(山东医科大学中草药学教研室)人参和西洋参相似,因国产西洋参生长年限短,根细、横纹少,又有全须西洋参的加工品种,故易与全须生晒人参相混。近年又对人参、西洋参的叶和果实开发利用,为对以上产品提...

【Abstract】 Paeoniflorins extracted by infusing, boiling and ultrasonic procedures from Sedative honey pill No. 2 and Radix Paeoniae Rubra were chromatographed on a C18 colunin with a mobile phase consisting of a mixture of methyl alcohol and 0. 05mol potassium dihydric phosphate (35 : 65 , pH3. 60) at a flow rate of 0. 8 ml/min , and detected by UV at 240nm. The linearity was excellent (r=0. 9998 ) within the range of 2~40 μg/ml of paeoniflorin. The intra-and inter-day coefficients of variation were 2. 25/00 (n= 6) and 2. 46 % (n = 6 ) respectively. The retention time(tR ) for paeoniflorin was 6.10 min under these conditions. Sample injection occurred every 12min. This method is simple, accurate and reproducible, and suitable for the quality control of honey pills as Sedative Honey Pill No. 2 and of Radix Paeoniae Rubra.

  • 【文献出处】 山东医科大学学报 ,ACTA ACADEMIAE MEDICINAE SHANDONG , 编辑部邮箱 ,1996年04期
  • 【分类号】R286
  • 【被引频次】6
  • 【下载频次】165
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