【摘要】 选择对草鱼出血病病毒（ＦＲＶ）不敏感的草鱼肝组织进行细胞培养，建立了草鱼肝细胞株，命名为ＧＬＡ．对ＧＬＡ进行攻毒，发现其对ＦＲＶ有抗性。采用电融合结合继代移核的方法，将ＧＬＡ细胞（核），移植于草鱼未受精卵内，获得开口前的幼鱼８尾和存活仔鱼１尾，说明此方法能提高细胞的发育能力。通过比较体细胞工程鱼与供体、受体亲本草鱼的形态特征，发现体细胞工程鱼可数性状与供体草鱼的基本一致，初步推断体细胞工程鱼的遗传信息，是由供体细胞核提供的。更多还原

【Abstract】 The somatic cell from grass carp liver cell strain (GLA) Which is resistant to grasscarp hemorhage virus (FRV) and which also has the same chromsome number as that of grass carp wastransferred into unfertilized eggs of grass carp by means of electric fusion. When the treated eggsdevelop at blastula stage, the cell from blastula was isolated and microinjected into unfertilized eggs ofgrass carp again. Using the above methods the cell-engineering grass carp was produced, and eight frybefore mouth - open stage and one big sized fingerling were obtained. The morphological studiesshown that no differences were found between cell-engineeing fish and donor grass carp, whichsuggested that nucleus of cell-engineering grass carp may possibly originate from somatic cell of GLA.This study also suggested that the technique may have the potential to produce cell-engineering fish foraquasulture and basic biological research. Further study on disease resistance of cell-engineering fish toFRV will be conducted.更多还原