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McAb-RIHA和McAb-ELISA检测急性血吸虫病患者尿排泄抗原的效果比较
COMPARATIVESTUDIES ON THE DETECTING CAg IN URINE OF ACUTE SCHISTOSOMIASIS BY McAb-RIHA AND McAb-Dot-ELISA
【摘要】 本研究建立了冰冻-加热浓缩法,将尿样浓缩20倍。应用McAb-RIHA和McAb-Dot-ELISA平行对比检测了83份急性血吸虫病患者和101份健康人尿样,两法的阳性检出率分别为78.31%和65.06%;假阳性率分别为14.85%和0%。就用McAb-RIHA和McAb-Dot-ELISA对比检查55份华枝睾吸虫病患者和49份钩虫病患者尿样,前者出现的交叉反应率分别为16.36%和14.28%;后者出现的交叉反应率均为0%。
【Abstract】 In the report, frozen-heat concentration was established firstly. Theoriginal urine was given a 20-fold concentration. McAb-RIHA AND McAb-Dot- ELISA were positive in 78.31% and 65.06% respectively, of 83patients with acute schistosomiasis. The false positive rates in 101healthy controls were 14.58% and 0% respectively. Cross reactions (using McAb-RIHA) were seen in 16.36% and 14.28% Of 55 patients withclonorchiasis. Corresponding figures for McAb-Dot-ELISA were 0 and 0%.
【关键词】 尿循环抗原;
急性血吸虫病;
McAb-RIHA;
McAb-Dot-ELISA;
【Key words】 Circulating antigen in urine; Acute schistosomiasis; McAb-RIHA; McAb-Dot-ELISA;
【Key words】 Circulating antigen in urine; Acute schistosomiasis; McAb-RIHA; McAb-Dot-ELISA;
【基金】 JRMC资助课题
- 【文献出处】 寄生虫病防治与研究 , 编辑部邮箱 ,1996年03期
- 【分类号】R532.21
- 【下载频次】11