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喜马拉雅旱獭G带核型研究

STUDY OF G-BANDED KARYOTYPE OF MARMOTA HIMALAYANA

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【作者】 尹敬如; 朱慧君; 王文青; 杨生玺; 李芳; 黄孝龙; 张昱;

【Author】 Yin Jingru;Zhu Huijun;Wang Wengqing; Yang Shengxi; Li Fang; Zhangyu.(Department of Biology,Qinghai Medical College)Huang Xiaolong (Qinghai Institute of Endemic Diseases)

【机构】 青海省地方病防治研究所!湟中811602;

【摘要】 本文采用外周血淋巴细胞培养技术和胰酶C-带法,对喜马拉雅旱獭染色体进行了G-带核型研究。通过雌雄7只旱獭80个G-带细胞的观察,均能看到各对染色体所具有的特征带型。按喜马拉雅旱獭核型2n=38[1]剪排了G-带带型[图1、图2]见封二。并绘制出G-带核型模式图[图3]。参照人类细胞遗传学命名法的国际体制,描述了各号染色体的带型特征。为旱獭属动物的染色体研究积累了资料,进而探讨该类动物的分类及系统演化提供细胞遗传学依据。

【Abstract】 The G-banded chromosomes of Marmota himalayana have been studied by the method of peripheral Lymphocyte culturc and pancreatic protease.Having observed 80 G-banding cells of 7 Marmota himalayana (3 males and 4 females) it shows that each homologous pair has its own special G-banded patterns,so it is easy to distinguish the chromosomes in pairs exactly in the Karyotype. According to the Karyotype formula 2n= 38,the idiogram of G-banded Karyotype was depicted.The X chromosome is similar to No. 10 chromosome in size, but it is different in arm length and banding pattern, therefore,it can be distinguishcd easily.The Y chromosome is the smallest and dyed darkly,so its banding patterns are hard to be recognized.

【关键词】 喜马拉雅旱獭; G-带; 染色体; 核型;
【Key words】 Marmota himalayana; G-banded Chromosome; karyotype;
  • 【文献出处】 青海医学院学报 ,Journal of Qinghai Medical College , 编辑部邮箱 ,1996年04期
  • 【分类号】S865
  • 【被引频次】4
  • 【下载频次】27
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