【摘要】 利用Ｓｉ－５色谱柱，石油醚／乙醇（７：３体积）流动相，检测波长３００纳米，成功地分离了２－（４－氯苯基）－３－甲基丁酸与（Ｓ）－α－苯乙胺生成的一对非对映体。分别测其线性关系及响应因子，由此计算出２－（４－氟苯基）－３－甲基丁酸的光学纯度。准确度为９９．２～１０１．０％，平均标准偏差为０．０６６％。更多还原

【Abstract】 A determination method for enantiomeric purity of 2- (4-chlorophenyl)-3-methylbutanoic acid via diastereomeric amide formation by HPLC was developed, using Si-5 column,petroleum and ethanol(7: 3 volume) as eluent, UV detector (235mn). The method is convenient, rapid and accurate. The accuracy was between 99. 0～ 101. 0%, and the average deviation was 0. 066.更多还原